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 Indian J Med Microbiol  
 

Figure 8: Masson's Trichrome staining for collagen deposits. a, b, c) The hPDL cells cultured in the presence of BioCaS cement at 7, 14 and 21 days. d, e, f) The hPDL cells cultured in the presence of sintered hydroxyapatite at 7, 14, and 21 days. g, h, i) hPDL cells in osteogenic induction medium at 7, 14 and 21 days (positive control) j, k, l) hPDL cells in regular medium at 7, 14, and 21 days (negative control) Collagen deposits are stained bluish green in the BioCaS group and positive control. (b and h at 14 days; c and i at 21 days. Collagen deposits were absent in HA group and negative control

Figure 8: Masson's Trichrome staining for collagen deposits. a, b, c) The hPDL cells cultured in the presence of BioCaS cement at 7, 14 and 21 days. d, e, f) The hPDL cells cultured in the presence of sintered hydroxyapatite at 7, 14, and 21 days. g, h, i) hPDL cells in osteogenic induction medium at 7, 14 and 21 days (positive control) j, k, l) hPDL cells in regular medium at 7, 14, and 21 days (negative control) Collagen deposits are stained bluish green in the BioCaS group and positive control. (b and h at 14 days; c and i at 21 days. Collagen deposits were absent in HA group and negative control