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 Indian J Med Microbiol  
 

Figure 7: Von Kossa staining for mineral deposits. a, b, c) The hPDL cells cultured in the presence of BioCaS cement at 7, 14 and 21 days. d, e, f) The hPDL cells cultured in the presence of sintered hydroxyapatite at 7, 14, and 21 days. g, h, i) hPDL cells in osteogenic induction medium at 7, 14 and 21 days (positive control) j, k, l) hPDL cells in regular medium at 7, 14, and 21 days (negative control). Von Kossa staining of hPDL cells showing brownish black deposits, which is evident in the BioCaS group- a, b and c at 7,14, and 21 days, respectively. Mineral deposits in the positive control are evident at 14 and 21 days (h, i). HA group and negative control did not show much mineral deposits

Figure 7: Von Kossa staining for mineral deposits. a, b, c) The hPDL cells cultured in the presence of BioCaS cement at 7, 14 and 21 days. d, e, f) The hPDL cells cultured in the presence of sintered hydroxyapatite at 7, 14, and 21 days. g, h, i) hPDL cells in osteogenic induction medium at 7, 14 and 21 days (positive control) j, k, l) hPDL cells in regular medium at 7, 14, and 21 days (negative control). Von Kossa staining of hPDL cells showing brownish black deposits, which is evident in the BioCaS group- a, b and c at 7,14, and 21 days, respectively. Mineral deposits in the positive control are evident at 14 and 21 days (h, i). HA group and negative control did not show much mineral deposits