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 Indian J Med Microbiol  
 

Figure 5: Alizarin red staining of hPDL cells. a, b, c) The hPDL cells cultured in the presence of BioCaS cement at 7, 14, and 21 days. d, e, f) The hPDL cells cultured in the presence of sintered hydroxyapatite at 7, 14, and 21 days. g, h, i) hPDL cells in osteogenic induction medium at 7, 14, and 21 days (positive control) j, k, l) hPDL cells in regular medium at 7, 14, and 21 days (negative control) The hPDL cells cultured in the presence of BioCaS cement showed early mineralization at 7 days. (a) Mineralization was evident in the form of nodules at 14 and 21 days (b and c respectively) in comparison to the positive control (h and i). The HA group and negative control showed little mineralization (d, e, f and j, k, l), respectively

Figure 5: Alizarin red staining of hPDL cells. a, b, c) The hPDL cells cultured in the presence of BioCaS cement at 7, 14, and 21 days. d, e, f) The hPDL cells cultured in the presence of sintered hydroxyapatite at 7, 14, and 21 days. g, h, i) hPDL cells in osteogenic induction medium at 7, 14, and 21 days (positive control) j, k, l) hPDL cells in regular medium at 7, 14, and 21 days (negative control) The hPDL cells cultured in the presence of BioCaS cement showed early mineralization at 7 days. (a) Mineralization was evident in the form of nodules at 14 and 21 days (b and c respectively) in comparison to the positive control (h and i). The HA group and negative control showed little mineralization (d, e, f and j, k, l), respectively