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 Indian J Med Microbiol  
 

Figure 8: Live P gingivalis increased TIMP-1 and TIMP-2 protein production by osteoblasts. Following osteoblast culture in the presence or absence of live and heat-inactivated P gingivalis (107 bacteria), cultured supernatants were collected and used to quantify TIMP-1 and TIMP-2. Culture medium was fi ltered through 0.22 ým fi lters and used to determine the amount of the secreted TIMP-1 and TIMP-2 using ELISA kits. Data are the means ▒ SD of four separate experiments. The levels of signifi cance relate to the differences between the values obtained from the P gingivalis-infected and noninfected cultures

Figure 8: Live P gingivalis increased TIMP-1 and TIMP-2 protein production by osteoblasts. Following osteoblast culture in the presence or absence of live and heat-inactivated P gingivalis (107 bacteria), cultured supernatants were collected and used to quantify TIMP-1 and TIMP-2. Culture medium was fi ltered through 0.22 ým fi lters and used to determine the amount of the secreted TIMP-1 and TIMP-2 using ELISA kits. Data are the means ▒ SD of four separate experiments. The levels of signifi cance relate to the differences between the values obtained from the P gingivalis-infected and noninfected cultures