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 Indian J Med Microbiol  
 

Figure 7: P gingivalis upregulated TIMP-1 and TIMP-2 mRNA expression. Following osteoblast culture in the presence or absence of live and heat-inactivated P gingivalis (107 bacteria), total RNA was extracted from the osteoblasts and RT-PCRs were performed using specifi c primers for TIMP-1, TIMP-2, and GAPDH. Changes in mRNA levels were measured using the public domain NIH Image software package. P gingivalisinfected cultures and noninfected cultures (CTRL) were compared. CTRL represents 100% gene activation and was used to determine the relative expression levels of TIMPs mRNA. Photos are of representative gels of three separate experiments. Histograms are mean SD of three separate experiments

Figure 7: P gingivalis upregulated TIMP-1 and TIMP-2 mRNA expression. Following osteoblast culture in the presence or absence of live and heat-inactivated P gingivalis (107 bacteria), total RNA was extracted from the osteoblasts and RT-PCRs were performed using specifi c primers for TIMP-1, TIMP-2, and GAPDH. Changes in mRNA levels were measured using the public domain NIH Image software package. P gingivalisinfected cultures and noninfected cultures (CTRL) were compared. CTRL represents 100% gene activation and was used to determine the relative expression levels of TIMPs mRNA. Photos are of representative gels of three separate experiments. Histograms are mean  SD of three separate experiments