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 Indian J Med Microbiol  
 

Figure 5: P gingivalis-modulated MMP mRNA expression. Following osteoblast culture in the presence or absence of live and heat-inactivated P gingivalis (107 bacteria), total RNA was extracted from the osteoblasts and RT-PCRs were performed using specifi c primers for MMP-2, MMP-9 and GAPDH. Changes in mRNA levels were measured using the public domain NIH Image software package. P gingivalis infected cultures and noninfected cultures (CTRL) were compared. CTRL represents 100% gene activation and was used to determine the relative expression levels of MMP mRNA. Photos are of representative gels of three separate experiments. Histograms are mean ± SD of three separate experiments

Figure 5: P gingivalis-modulated MMP mRNA expression. Following osteoblast culture in the presence or absence of live and heat-inactivated P gingivalis (107 bacteria), total RNA was extracted from the osteoblasts and RT-PCRs were performed using specifi c primers for MMP-2, MMP-9 and GAPDH. Changes in mRNA levels were measured using the public domain NIH Image software package. P gingivalis infected cultures and noninfected cultures (CTRL) were compared. CTRL represents 100% gene activation and was used to determine the relative expression levels of MMP mRNA. Photos are of representative gels of three separate experiments. Histograms are mean ± SD of three separate experiments