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 Indian J Med Microbiol  
 

Figure 1: Effect of P gingivalis on osteoblast attachment and growth. Osteoblasts were seeded into six-well plates and cultured at 37C in a humid 5% CO2 atmosphere. When the culture reached 80% confl uence, the cells were either not infected or infected with live or heat-inactivated P gingivalis at various concentrations. After 24 h, the cells were observed, photographed, and subjected to a subsequent MTT assay. The photographs (200 magnifi cation) are representative of six separate experiments. The histograms were created using the mean relative values SD for 10 separate experiments. Differences in osteoblast growth in the presence and absence of P gingivalis were considered signifi cant at P < 0.05 or less

Figure 1: Effect of P gingivalis on osteoblast attachment and growth. Osteoblasts were seeded into six-well plates and cultured at 37C in a humid 5% CO2 atmosphere. When the culture reached 80% confl uence, the cells were either not infected or infected with live or heat-inactivated P gingivalis at various concentrations. After 24 h, the cells were observed, photographed, and subjected to a subsequent MTT assay. The photographs (200 magnifi cation) are representative of six separate experiments. The histograms were created using the mean relative values  SD for 10 separate experiments. Differences in osteoblast growth in the presence and absence of P gingivalis were considered signifi cant at P < 0.05 or less