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 Indian J Med Microbiol  
 

Figure 1: Effect of P gingivalis on osteoblast attachment and growth. Osteoblasts were seeded into six-well plates and cultured at 37°C in a humid 5% CO2 atmosphere. When the culture reached 80% confl uence, the cells were either not infected or infected with live or heat-inactivated P gingivalis at various concentrations. After 24 h, the cells were observed, photographed, and subjected to a subsequent MTT assay. The photographs (200× magnifi cation) are representative of six separate experiments. The histograms were created using the mean relative values ± SD for 10 separate experiments. Differences in osteoblast growth in the presence and absence of P gingivalis were considered signifi cant at P < 0.05 or less

Figure 1: Effect of P gingivalis on osteoblast attachment and growth. Osteoblasts were seeded into six-well plates and cultured at 37°C in a humid 5% CO2 atmosphere. When the culture reached 80% confl uence, the cells were either not infected or infected with live or heat-inactivated P gingivalis at various concentrations. After 24 h, the cells were observed, photographed, and subjected to a subsequent MTT assay. The photographs (200× magnifi cation) are representative of six separate experiments. The histograms were created using the mean relative values ± SD for 10 separate experiments. Differences in osteoblast growth in the presence and absence of P gingivalis were considered signifi cant at P < 0.05 or less