Indian Journal of Dental Research

ORIGINAL RESEARCH
Year
: 2020  |  Volume : 31  |  Issue : 4  |  Page : 562--568

Sex determination from the pulp tissue of deciduous teeth exposed to natural soil and wet clay - A PCR study


Prachi Suman, R Manju, Veena A Shetty, Amitha M Hegde, Muthtamil, Shama Rao 
 NITTE (Deemed to be University), AB Shetty Memorial Institute of Dental Sciences, Department of Pediatric and Preventive Dentistry, Deralakatte, Mangalore, Karnataka, India

Correspondence Address:
Dr. R Manju
NITTE (Deemed to be University), AB Shetty Memorial Institute of Dental Sciences, Department of Pediatric and Preventive Dentistry, Deralakatte, Mangalore, Karnataka
India

Context: Dental tissue remains are the toughest, and chemically, the most stable tissue in the body. Its high resilience in the events of fire and bacterial decomposition makes them vital for DNA analysis by PCR method. Aims: Determination of sex of children through molecular analysis of pulp tissue of exfoliated deciduous teeth stored in different media and analyzed after a different time period. Settings and Design: Sixty samples of deciduous teeth were divided into three groups. Group IA and Group IIA were stored in natural soil and wet clay for 1 month, respectively. Group IB and Group IIB were stored in natural soil and wet clay for 6 months, respectively. Group III was analyzed immediately after extraction. Methods and Material: Sex determination was carried out in five steps: Pulp tissue removal, DNA isolation, DNA quantification, PCR amplification, Sex determination. X and Y specific chromosomes from each sample were amplified and compared. Statistical Analysis Used: Kruskal-Wallis test, Dunn's test, and Wilcoxon signed rank test. Results: Group III revealed the highest amount of DNA quantified. Amount of DNA quantified after 6 months of storage in natural soil and wet clay decreased in both the groups with the samples stored in wet clay showing a maximum decrease. Results of the PCR analysis also showed 100% accuracy rate in the samples of Group III. Conclusions: Sex determination from pulp tissue depends a lot on the quality and quantity of DNA extracted. Sex could be effectively determined among the samples evaluated immediately after extraction. This ability decreases as the storage condition changes and the time period increases. Samples stored in wet clay were found to show the least sex identification ability than dry soil.


How to cite this article:
Suman P, Manju R, Shetty VA, Hegde AM, Muthtamil, Rao S. Sex determination from the pulp tissue of deciduous teeth exposed to natural soil and wet clay - A PCR study.Indian J Dent Res 2020;31:562-568


How to cite this URL:
Suman P, Manju R, Shetty VA, Hegde AM, Muthtamil, Rao S. Sex determination from the pulp tissue of deciduous teeth exposed to natural soil and wet clay - A PCR study. Indian J Dent Res [serial online] 2020 [cited 2020 Dec 2 ];31:562-568
Available from: https://www.ijdr.in/article.asp?issn=0970-9290;year=2020;volume=31;issue=4;spage=562;epage=568;aulast=Suman;type=0