Indian Journal of Dental Research

ORIGINAL RESEARCH
Year
: 2018  |  Volume : 29  |  Issue : 4  |  Page : 470--476

Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction


Ripon Md Chowdhury1, Abhishek Singhvi2, Neeta Bagul2, Sanya Bhatia2, Gurdeep Singh2, Surajit Goswami3 
1 Department of Oral and Maxillofacial Pathology, Mithila Minority Dental College and Hospital, Laheriasarai, Darbhanga, Bihar, India
2 Department of Oral and Maxillofacial Pathology, Vyas Dental College, Jodhpur, Rajasthan, India
3 Medinova, West Bengal, India

Correspondence Address:
Dr. Ripon Md Chowdhury
Department of Oral and Maxillofacial Pathology, Mithila Minority Dental College and Hospital, Ekmighat, Mansukh Nagar, Laheriasarai, Darbhanga - 864 001, Bihar
India

Introduction: Forensic odontology necessarily involves the application of dentistry along with various other branches of sciences which deals with proper handling, examination, evaluation, and presentation of dental evidences, that aids to investigate a crime and deliver justice. Sex determination is a part of forensic odontology and an essential priority when traditional identification of the deceased becomes impossible. Aim: To determine Sex by analysis of the Amelogenin gene using Polymerase Chain Reaction (PCR) method on Deoxyribose nucleic acid (DNA) isolated from dental pulp, which was exposed to various environmental conditions created artificially to mimic a forensic scenario. Materials and Method: This in-vitro study was conducted by subjecting extracted teeth to various conditions imitating a forensic scene, viz. desiccation at room temperatures, immersion in salt water, burial in soil and even exposing to extremes of temperatures. DNA was extracted from dental pulp tissue and sex determination was achieved by amplification of the amelogenin gene through AMEL gene based primers in PCR. Result: Among all the samples used in this study, DNA could be extracted from all, except from those that were subjected to a temperature of 350 °C. DNA amplification and sex determination of the samples were found to be accurate when compared to sex of the individual which was recorded initially, during collection of teeth samples. Conclusion: This study shows teeth to be a potent source of DNA even in extreme environmental conditions, barring high temperatures and determination of sex by PCR amplification of AMEL markers to be quite reliable.


How to cite this article:
Chowdhury RM, Singhvi A, Bagul N, Bhatia S, Singh G, Goswami S. Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction.Indian J Dent Res 2018;29:470-476


How to cite this URL:
Chowdhury RM, Singhvi A, Bagul N, Bhatia S, Singh G, Goswami S. Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction. Indian J Dent Res [serial online] 2018 [cited 2021 Feb 25 ];29:470-476
Available from: https://www.ijdr.in/article.asp?issn=0970-9290;year=2018;volume=29;issue=4;spage=470;epage=476;aulast=Chowdhury;type=0