Indian Journal of Dental Research

ORIGINAL RESEARCH
Year
: 2017  |  Volume : 28  |  Issue : 2  |  Page : 138--143

Screening the antigenic properties of Porphyromonas gingivalis and level of raised antibodies after antigenic injection into Balb/c Mice


Mahdi T Khadematolrasoul1, Farin Kiany2, Faezeh Saadati1 
1 Postgraduate Student, Department of Periodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
2 Oral and Dental Disease Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence Address:
Faezeh Saadati
Department of Periodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz
Iran

Background: Different diagnostic screening tests have been developed to detect periodontal disease in the early stages. Despite these advances still, there is a need for a more practical and beneficial diagnostic test. Aim: The aim of this study was to investigate the possibility of developing such a kit based on the body immune response against Porphyromonas gingivalis. Method and Materials: This experimental study was conducted by culturing P. gingivalis and extracting its antigens. These antigens were injected into peritoneal cavity of four Balb/c mice. Finally, the pattern, type, and quantity of antibody response against P. gingivalis antigen were detected. Results of the study showed that 3.0 × 108 cells of P. gingivalis are an appropriate count for stimulating the immunization in Balb/c mice and the subsequent amount of antibody (IgG) production was 81.5 μg/ml. Result: The antigenic injections which were done in the current study could mimic the condition of periodontal disease and the raise of P. gingivalis in the body. Conclusion: The obtained data can be used in future attempts to develop practical and usable test kits against P. gingivalis.


How to cite this article:
Khadematolrasoul MT, Kiany F, Saadati F. Screening the antigenic properties of Porphyromonas gingivalis and level of raised antibodies after antigenic injection into Balb/c Mice.Indian J Dent Res 2017;28:138-143


How to cite this URL:
Khadematolrasoul MT, Kiany F, Saadati F. Screening the antigenic properties of Porphyromonas gingivalis and level of raised antibodies after antigenic injection into Balb/c Mice. Indian J Dent Res [serial online] 2017 [cited 2021 Nov 30 ];28:138-143
Available from: https://www.ijdr.in/article.asp?issn=0970-9290;year=2017;volume=28;issue=2;spage=138;epage=143;aulast=Khadematolrasoul;type=0