Indian Journal of Dental ResearchIndian Journal of Dental ResearchIndian Journal of Dental Research
HOME | ABOUT US | EDITORIAL BOARD | AHEAD OF PRINT | CURRENT ISSUE | ARCHIVES | INSTRUCTIONS | SUBSCRIBE | ADVERTISE | CONTACT
Indian Journal of Dental Research   Login   |  Users online:

Home Bookmark this page Print this page Email this page Small font sizeDefault font size Increase font size         

 


 
ORIGINAL RESEARCH Table of Contents   
Year : 2020  |  Volume : 31  |  Issue : 6  |  Page : 916-920
Antimicrobial Capacity of a Hydroxyapatite–Lysozyme–Lactoferrin–Lactoperoxidase Combination Against Streptococcus mutans for the Treatment of Dentinal Caries


1 Department of Dentistry, Master's Program in Health Sciences, Pontificia Universidade Catolica de Campinas (PUC-Campinas), Campinas, SP, Brazil
2 Department of Dentistry, PUC-Campinas, Campinas, SP, Brazil

Correspondence Address:
Prof. Sérgio L Pinheiro
Rua das Quaresmeiras, 54, Residencial Jardim das Palmeiras, Vinhedo - 13284-503, SP
Brazil
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijdr.IJDR_474_18

Rights and Permissions

Context: Hydroxyapatite has shown to regenerate the mineralized layer of dentin, whereas the combination of the enzymes lysozyme, lactoferrin, and lactoperoxidase may exhibit antimicrobial properties against oral pathogens. Aims: To evaluate a combination of hydroxyapatite and lysozyme, lactoferrin, and lactoperoxidase for the treatment of dentinal caries by measuring viable Streptococcus mutans. Settings and Design: Laboratory study with experimental groups. Methods and Material: Carious lesions in 20 permanent third molars were treated with a combination of hydroxyapatite and the enzymes lysozyme, lactoferrin, and lactoperoxidase. Carious dentin was collected and homogenized in a vortex shaker. After homogenization, five decimal dilutions were performed. Three aliquots of 25 μL of each dilution were seeded onto the surface of mitis salivarius bacitracin (MSB) medium. All plates were incubated in anaerobic jars. After incubation, the viable bacterial count was determined. S. mutans counts were obtained before and 24 h, 1 month, and 6 months after treatment. Statistical Analysis Used: Descriptive statistical analysis and the Kruskal–Wallis test, supplemented by the Student–Newman–Keuls test. Results: A significant reduction in S. mutans counts was observed 24 h after sealing with a combination of hydroxyapatite, lysozyme, lactoferrin, and lactoperoxidase as compared to counts after 1 month and after 6 months (P < 0.05). Conclusions: The combination of hydroxyapatite with lysozyme, lactoferrin, and lactoperoxidase may be an alternative for S. mutans control in dentinal caries.


[FULL TEXT] [PDF]*
Print this article     Email this article

 
 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
  Citation Manager
 Access Statistics
  Reader Comments
  Email Alert *
  Add to My List *
 
 

 Article Access Statistics
    Viewed912    
    Printed54    
    Emailed0    
    PDF Downloaded39    
    Comments [Add]    

Recommend this journal