| |
|
|
| Year : 2010 | Volume
: 21
| Issue : 3 | Page : 380-384 |
|
| Chitosan-based polyherbal toothpaste: As novel oral hygiene product |
|
|
Nitin C Mohire, Adhikrao V Yadav
Department of Pharmacy, Government College of Pharmacy, Vidyanagar, Karad, District- Satara, Maharashtra, India
Click here for correspondence address and email
| Date of Submission | 25-Oct-2009 |
| Date of Decision | 06-Jan-2010 |
| Date of Acceptance | 21-May-2010 |
| Date of Web Publication | 29-Sep-2010 |
|
|
 |
|
 Abstract | | |
Objective: The objective of the present work was to develop chitosan-based polyherbal toothpaste and evaluate its plaque-reducing potential and efficacy in reduction of dental pathogens.
Materials and Methods: Antimicrobial activity of herbal extracts against dental pathogens were performed by using disk diffusion method. The pharmaceutical evaluation of toothpaste was carried out as per the US Government Tooth Paste Specifications. A 4-week clinical study was conducted in patients with oro-dental problems to evaluate the plaque removing efficacy of chitosan-based polyherbal toothpaste with commercially available chlorhexidine gluconate (0.2% w/v) mouthwash as positive control. Total microbial count was carried out to determine the percentage decrease in the oral bacterial count over the period of treatment.
Result: Herbal extracts were found to possess satisfactory antimicrobial activity against most of the dental pathogens. Chitosan-containing polyherbal toothpaste significantly reduces the plaque index by 70.47% and bacterial count by 85.29%, and thus fulfills the majority of esthetic and medicinal requirements of oral hygiene products.
Conclusion: Chitosan-based polyherbal toothpaste proves itself as a promising novel oral hygiene product as compared with currently available oral hygiene products. A further study to confirm the exact mechanism and active constituents behind antiplaque and antimicrobial activity of chitosan-based polyherbal toothpaste and its efficacy in large number of patient population is on high demand. Keywords: Chitosan, chlorhexidine gluconate, oral hygiene, plaque index, toothpaste
How to cite this article:
Mohire NC, Yadav AV. Chitosan-based polyherbal toothpaste: As novel oral hygiene product. Indian J Dent Res 2010;21:380-4 |
How to cite this URL:
Mohire NC, Yadav AV. Chitosan-based polyherbal toothpaste: As novel oral hygiene product. Indian J Dent Res [serial online] 2010 [cited 2023 Jan 27];21:380-4. Available from: https://www.ijdr.in/text.asp?2010/21/3/380/70808 |
Dentifrices and mouthwashes that are generally produced by cosmetic manufacturers are really not cosmetics. They fall more properly into the field of hygienic products just as soap. Both products have more or less the same function, which are to cleanse, to counteract bad breath, and to leave a refreshing clean taste in the mouth. It may also be argued that properly cleaned teeth add to personal appearance and good health. [1] Good oral hygiene results in a mouth that looks and smells healthy. This means, teeth are clean and free of debris, gums are pink and do not hurt or bleed when you brush or floss, and bad breath is not a constant problem. [2]
Oral hygiene is of utmost importance as it contributes in esthetic expectations of an individual and of society. The strong positive association that exists between poor oral hygiene and gingival and periodontal diseases makes poor hygiene the primary etiologic agent. [3]
We have already reported antimicrobial activity of chitosan. [4] Chitosan, an abundant natural polymer, is obtained by alkaline N-deacetylation of chitin. Chitosan being a binding agent, bio-adhesive, bio-compatible, bio-degradable, and non-toxic polymer also possessing medicinal activities, such as antifungal, antibacterial, antiprotozoal, anticancer, antiplaque, antitartar, hemostatic, wound healing, and potentiates anti-inflammatory response, inhibits the growth of cariogenic bacteria, immunopotentiation, antihypertensive, serum cholesterol lowering, absorption enhancer, increases salivary secretion (anti-xerostomial), and helps in the formation of bone substitute materials. [5],[6],[7]
The adherence of oral bacteria on the tooth surface leads to plaque formation. It is believed that the adhesion between the bacteria and the tooth surface is due to electrostatic and hydrophobic interactions. These interactions are disrupted by chitosan derivatives because of competition by the positively charged amine group. [8],[9] The antibacterial activity of chitosan could be due to the electrostatic interactions between the amine groups of chitosan and the anionic sites on bacterial cell wall because of the presence of carboxylic acid residues and phospholipids. [10]
Use of most of the currently used gelling agents, such as tragacanth, Irish moss, and sodium alginate mucilage, in the toothpaste was limited only to their gelling capacity and also require antimicrobial preservatives due to their carbohydrate nature, whereas chitosan being a good gelling agent, does not require any preservatives as chitosan possess antimicrobial activities.
The antimicrobial activity of Pterocarpus marsupium aqueous extract (PM), Stevia rebaudiana aqueous extract (SR), Glycyrrhiza glabra aqueous extract (GG) against selected dental pathogens was carried out at our research center to support the study.
Chlorhexidine gluconate (CHX) 0.2% solution is still the golden standard as mouthrinse for the prevention of plaque formation and development of gingivitis. CHX has good antimicrobial activity against the microbes responsible for oral infections. [11] Although antimicrobial effect of CHX is generally undisputed and well acknowledged, the mode of treatment and delivery system for maximal effectiveness is not yet fully clear. The side effects associated with CHX, such as extrinsic tooth staining, poor taste, taste disturbance, sensitivity changes in tongue, pain, and the content of alcohol led us to search for a novel oral hygiene product.
Considering the limitations of the presently available products and the promising properties of chitosan and selected herbs suitable to address oral care problems, present invention was directed to design and develop chitosan-based polyherbal toothpastes with enhanced performance in oral care as chitosan inhibits the growth of Streptococcus mutans  class="ref" name="ft12" href="#ref12">[12] and Porphyromonas gingivalis; [13] microorganisms responsible for caries and gingivitis, respectively. Chitosan also potentiates the effectiveness of active ingredients of toothpaste for antimicrobial and anti-inflammatory activities.
| Materials and Methods | |  |
Materials
All the chemicals used are of analytical grade. Precipitated calcium carbonate, Dicalcium phosphate, Gum tragacanth, Sodium lauryl sulfate, Sorbitol, and Eugenol were purchased from Loba chemie, Mumbai. Silicon dioxide was received as a gift sample from Okasa Pharma, Satara. Chitosan was received as a gift sample from India See Food, Cochin, Kerala.
Methods
PM, GG, and SR, were prepared in various strengths: 50, 100, 500 μg/mL and their antimicrobial activity against selected dental pathogens, such as Pseudomonas aueroginosa, Proteus vulgaris, Escherichia More Details coli, Bacillus subtilis, Lactobacillus acidophilus, Candida albicans were determined by using disk diffusion method as mentioned in [Figure 1]. Gentamycin 100 μg/mL was used as standard for evaluation of antimicrobial activity and fluconazole 10 μg/mL, was used as standard for evaluation of antifungal activity. | Figure 1 :Antimicrobial activity of herbal extracts against dental pathogens
Click here to view |
Two toothpaste formulations (Test (Polyherbal), Placebo) were prepared and compared with CHX (0.2%) mouthwash for its clinical efficacy.
Test (Polyherbal) toothpaste: Chitosan gel and Sorbitol were mixed thoroughly. Abrasives, such as Precipitated calcium carbonate, Dicalcium phosphate, and other excipients were added and mixed in mortar and pestle. Finely ground herbal extracts and Eugenol were added and after thorough mixing, the toothpastes were filled in the tubes.
Placebo toothpaste was prepared by the same method but do not contain chitosan, herbal extracts, and eugenol. Gum tragacanth mucilage was used as gelling agent instead of chitosan gel. Purpose of adding different ingredients in the toothpaste is mentioned in [Table 1].
Evaluation of toothpastes
(A) Pharmaceutical evaluation
The formulated toothpastes were evaluated as per the US Government Tooth Paste Specifications. [20] The results obtained are presented in [Table 2].
(B) Clinical evaluation
The clinical evaluation was carried out as per the protocol approved by Institutional Ethics Committee. Eighteen healthy human volunteers, whose informed consent was first obtained, were selected for the study.
The groups were treated as follows:
Group-I: Placebo: Toothpaste without chitosan and herbal ingredients.
Group-II: Positive control: CHX (0.2% w/v) mouthwash.
Group-III: Test (Polyherbal): Toothpaste with chitosan, eugenol, and extracts of PM, SR, and GG.
(a) Clinical evaluation to study antiplaque activity
The study involved 18 subjects who underwent inclusion/exclusion criteria were divided into 3 groups containing 6 members in each group. Subjects were distributed in such a way that the average baseline plaque index of each group remains fairly the same. The study consisted of assessing the baseline plaque index status according to the criteria given by Silness and Loe (1964). [21] The subjects were supplied with polyherbal toothpaste, chlorhexidine mouthwash, and placebo toothpaste for application for a period of 4 weeks. After 2 and 4 weeks of use of the assigned product, the examining dentist scored the subjects for plaque index. The same dentist was interpreted at each examination, to avoid inter-examiner variation. The scores of the plaque index are presented in [Table 3] and [Figure 2].
(b) Oral bacterial count from subjects
The subjects of each group were asked to simulate chewing action with sterile cotton rolls and asked to swallow the saliva thus collected over the next 1 min. This procedure was carried out in order to clear the mouth of any residual saliva. The subjects were then made to chew the cotton roll for next 4 min and expectorate into sterile bottles. Aseptically collected sample was further diluted and incubated on nutrient agar media. The percentage decrease in the total bacterial count in the study groups is presented in [Table 4] and [Figure 3]. | Table 4 :Percentage decrease in total bacterial count in study groups with±S.D
Click here to view |
 | Figure 3 :Percentage decrease in total bacterial count in study groups with±SD
Click here to view |
| Discussion | | |
Bacterial plaque accumulated on teeth surfaces and composed of native oral flora, is the primary etiologic agent for periodontal disease and dental caries, which may result in tooth loss if left untreated. [22],[23] S. mutans and lactobacilli are the microorganisms that are primarily associated with dental caries. [24]
S. mutans, P. gingivalis, P. aueroginosa, P. vulgaris, E. coli, B. subtilis, L. acidophilus, and C. albicans are the common dental pathogens found to be associated with most of the orodental problems. As chitosan possesses and potentiates inhibitory action against S. mutans and P. gingivalis, it is necessary to evaluate the antimicrobial activity of herbal extracts against the remaining dental pathogens. Thus antimicrobial activities of PM, SR, and GG at various strengths were determined against selected dental pathogens and found to be satisfactory for maintaining good oral hygiene.
Pharmaceutical evaluation of novel chitosan-based polyherbal toothpaste was found to be well complied with all the US Government Tooth Paste Specifications.
The clinical evaluation carried out in healthy human volunteers as per the protocol showed that human volunteers in the test (polyherbal) group had a significant decrease in both plaque index and decrease in bacterial count as compared with positive control and placebo group.
The reduction in plaque index is caused by the combined effect of the physical properties, such as mucoadhesion and chemical interactions of chitosan with bacterial cell wall and antimicrobial effect of herbal extracts and eugenol. The percentage decrease in total bacterial count in the study groups were again due to the use of chitosan and herbal extracts.
The slight decrease in the plaque index as well as bacterial count in the placebo group might be due to performing routine oral hygiene practices by the volunteers from this group. This indicates the importance of routine oral hygiene practices.
| Result and Conclusion | | |
Several toothpastes are currently being used as potential means of oral hygiene products. However, none of these fulfill the comprehensive requirements of oral care. The results of the study suggest that extracts of PM, SR, and GG possess satisfactory antimicrobial activity, which ultimately results in reducing total microbial count. Thus incorporation of chitosan as the gelling agent as well as therapeutic agent with herbal extracts in toothpaste reduces the plaque index by 70.47% and bacterial count by 85.29%, and thus fulfills the majority of esthetic and medicinal requirements of oral hygiene products.
From the results obtained in this study, it is concluded that chitosan-based polyherbal toothpaste proves itself as a promising oral hygiene product.
Future
Ultimate aim of every research is to benefit human beings. Thus further studies are needed to identify and purify active ingredients from herbal extracts for its future use in toothpastes, mouthrinse, and other oral hygiene products. In addition, long-term clinical studies in large number of patient population will be required to evaluate the usefulness of these materials more exactly. The actual mechanism involved behind antimicrobial activity of herbal extracts need to be researched so as to decide its dose and duration of therapy.
| Acknowledgments | | |
Authors are thankful to Dr. S.B. Bhise, Principal, Govt. College of Pharmacy, Karad, for providing all necessary facilities and Okasa Pharma, Satara, for providing gift samples. We kindly appreciate leading Dentist Dr. Jaiwant Sawant, Karad, and Vaishali Gaikwad for providing valuable support in the clinical evaluation. We are thankful to Rameshwardasji Birla Smarak Kosh for their financial support.
| References | | |
| 1. | Thomsen EG. Dentifrices and mouth washes. Modern cosmetics. Bombay: Universal Publishing Corporation Bombay; 1985. p. 443.  |
| 2. | Oral health: A window to your overall health by Colgate world of care and Mayoclinic. Com 2006. Available from: http://www.mayoclinic.com/health /dental/DE00001 [last cited on 2006 Dec 06]. |
| 3. | Carranza FA. Glickmann′s Clinical Periodontology. 7th ed. Los Angeles, California: WB Saunders Company University of California; 1990. p. 321. |
| 4. | Yadav AV, Bhise SB. Chitosan: A potential biomaterial effective against typhoid. Curr Sci 2004;87:1176-8. |
| 5. | Radi H, Mansoor A. Chitosan based gastrointestinal system. J Control Release 2003;89:151-65. |
| 6. | Yoshihiko H, Naoko O, Tomoko G, Keiji Y, Takahide I, Taisuke N, et al. Chewing chitosan containing gum effectively inhibits the growth of cariogenic bacteria. Arch Oral Biol 2007;52:290-4. |
| 7. | Chitosan from Wikipedia, free encyclopedia. Available from: http://www.en.wikipedia.org/wiki/Chitosan [last cited on 2008]. |
| 8. | Nesbitt WE, Doyle RJ, Tyler KG. Hydrophobic interactions and the adherence of Streptococcus sanguis to hydroxyapetite. Infect Immunol 1982;38:637-44. |
| 9. | Rolla G, Robrish SA, Bowen SH. Interactions of hydroxyapetite and protein coated hydroxyapetite with Streptococcus mutants and Streptococcus sanguis. Acta Pathol Microbiol Scand Sec 1997;5:341-6. |
| 10. | Seo H, Shoji A, Itoh Y, Kawamura M, Sakagami Y, Karnicki ZS, et al. Antibacterial fiber blended with chitosan. Chitin World, Wirtschaftverlag, Germany: 1994. p. 623-31. |
| 11. | Emilson CS. Potential efficacy of chlorhexidine against mutans Streptococci and human dental caries. J Dent Res 1994;73:682-91. |
| 12. | Fujiwara M, Hayashi Y, Ohara N. Inhibitory effect of water soluble chitosan on growth of Streptococcus mutans. New Microbiol 2004;27:83-6. [PUBMED] |
| 13. | Ikinci G, Senel S, Akincibay H, Kas S, Ercis S, Wilson CG, et al. Effect of chitosan on a periodontal pathogen Porphyromonas gingivalis. Int J Pharm 2002;235:121-7. |
| 14. | Dr Duke′s Phytochemical and Ethnobotanical Databases. Available from: http://www.ars-grin.gov/cgi-bin/duke/ethnobot.pl [last cited on 2008]. |
| 15. | Moynihan P. Food and dietary factors that prevent dental caries. Quintessence Int 2007;38:320-4. [PUBMED] |
| 16. | Liquorice from Wikipedia, free encyclopedia. Available from: http://www.en.wikipedia.org/wiki/Liquorice [last cited on 2008]. |
| 17. | Mohire NC, Salunkhe VR, Yadav AV, Bhise SB. Cardiotonic activity of aqueous extract of heartwood of Pterocarpus marsupium. Indian J Exp Biol 2007;45:532-7. |
| 18. | Mankani KL, Krishna V, Manjunatha BK, Vidya SM, Sing JK, Manohara YN, et al. Evaluation of wound healing property of Pterocarpus marsupium stem bark. Indian Drugs 2005;42:432-6. |
| 19. | Eugenol from Wikipedia, free encyclopedia. Available from: http://www.en.wikipedia.org/wiki/Eugenol [last cited on 2008]. |
| 20. | Thomsen EG. Dentifrices and mouth washes. Modern cosmetics. Bombay: Universal Publishing Corporation Bombay; 1985. p. 473-5. |
| 21. | Peter S. Essentials of preventive and community dentistry. 1st ed. Delhi: Arya (Medi) Publishing House; 2008 p. 471-3. |
| 22. | Gibbons RJ. Adherence interactions which may affect microbial etiology in the mouth. J Dent Res 1984;63:378-85. [PUBMED] [FULLTEXT] |
| 23. | Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50:353-80. [PUBMED] [FULLTEXT] |
| 24. | Mitchell L. Decalcification during orthodontic treatment with fixed appliances: An overview. Br J Orthod 1992;19:199-205. [PUBMED] |
Correspondence Address:
Nitin C Mohire
Department of Pharmacy, Government College of Pharmacy, Vidyanagar, Karad, District- Satara, Maharashtra
India
 Source of Support: Rameshwardasji Birla Smarak Kosh, Conflict of Interest: None  | Check |
DOI: 10.4103/0970-9290.70808
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4] |
|
| This article has been cited by | | 1 |
Chitosan and Carrageenan-Based Biocompatible Hydrogel Platforms for Cosmeceutical, Drug Delivery, and Biomedical Applications |
|
| Muhammad Anees ur Rehman Qureshi, Nasima Arshad, Atta Rasool, Atif Islam, Muhammad Rizwan, Muhammad Haseeb, Tahir Rasheed, Muhammad Bilal | | Starch - Stärke. 2022; : 2200052 | | [Pubmed] | [DOI] | | | 2 |
Chitosan-based drug delivery systems: current strategic design and potential application in human hard tissue repair |
|
| Zhenqi Liu, Kun Wang, Xiu Peng, Linglin Zhang | | European Polymer Journal. 2022; : 110979 | | [Pubmed] | [DOI] | | | 3 |
Evaluation of Fluoride Release in Chitosan-Modified Glass Ionomer Cements |
|
| Cruz Nishanthine, Revathi Miglani, Indira R, Saravanan Poorni, Manali Ramakrishnan Srinivasan, Ali Robaian, Nassreen Hassan Mohammad Albar, Susen Faisal Rajeh Alhaidary, Sultan Binalrimal, Abdullah Almalki, Thilla Sekar Vinothkumar, Harisha Dewan, Waseem Radwan, Mubashir Baig Mirza, Shilpa Bhandi, Shankargouda Patil | | International Dental Journal. 2022; | | [Pubmed] | [DOI] | | | 4 |
Role of chitosan in titanium coatings. trends and new generations of coatings |
|
| Nansi López-Valverde, Javier Aragoneses, Antonio López-Valverde, Cinthia Rodríguez, Bruno Macedo de Sousa, Juan Manuel Aragoneses | | Frontiers in Bioengineering and Biotechnology. 2022; 10 | | [Pubmed] | [DOI] | | | 5 |
Advancements in Fabrication and Application of Chitosan Composites in Implants and Dentistry: A Review |
|
| Fariborz Sharifianjazi, Samad Khaksar, Amirhossein Esmaeilkhanian, Leila Bazli, Sara Eskandarinezhad, Peyman Salahshour, Farnaz Sadeghi, Sadegh Rostamnia, Seyed Mohammad Vahdat | | Biomolecules. 2022; 12(2): 155 | | [Pubmed] | [DOI] | | | 6 |
Formulation and Evaluation of Herbal Toothpowder |
|
| Miss. Pratiksha N. Uchale, Dr. Subhash T. Kumbhar | | International Journal of Advanced Research in Science, Communication and Technology. 2022; : 59 | | [Pubmed] | [DOI] | | | 7 |
Efficacy of Chitosan and Chlorhexidine Mouthwash on Dental Plaque and Gingival Inflammation: A Systematic Review |
|
| Indumathy Pandiyan, Pradeep Kumar Rathinavelu, Meignana I Arumugham, Srisakthi D, Arthi Balasubramaniam | | Cureus. 2022; | | [Pubmed] | [DOI] | | | 8 |
Chitosan hybrids for cosmeceutical applications in skin, hair and dental care: an update |
|
| N. Sanoj Rejinold, Goeun Choi, Jin-Ho Choy | | Emergent Materials. 2021; 4(5): 1125 | | [Pubmed] | [DOI] | | | 9 |
Preparation and application of chitosan biomaterials in dentistry |
|
| Chenxi Zhang, Didi Hui, Colin Du, Huan Sun, Wei Peng, Xiaobing Pu, Zhengyong Li, Jianxun Sun, Changchun Zhou | | International Journal of Biological Macromolecules. 2021; 167: 1198 | | [Pubmed] | [DOI] | | | 10 |
Comparative evaluation of Stainless-steel wires and brackets coated with nanoparticles of Chitosan or Zinc oxide upon friction: An in vitro study |
|
| Nahla Elhelbawy, Mohammed Ellaithy | | International Orthodontics. 2021; 19(2): 274 | | [Pubmed] | [DOI] | | | 11 |
Effect of the Incorporation of Chitosan and TiO2 Nanoparticles on the Shear Bond Strength of an Orthodontic Adhesive: An In Vitro Study |
|
| Fahimeh Farzanegan, Hooman Shafaee, Majid Darroudi, Abdolrasoul Rangrazi | | Journal of Advanced Oral Research. 2021; 12(2): 261 | | [Pubmed] | [DOI] | | | 12 |
Effectiveness of herbal oral care products in reducing dental plaque & gingivitis – a systematic review and meta-analysis |
|
| Chandrashekar Janakiram, Ramanarayanan Venkitachalam, Paul Fontelo, Timothy J. Iafolla, Bruce A. Dye | | BMC Complementary Medicine and Therapies. 2020; 20(1) | | [Pubmed] | [DOI] | | | 13 |
Preparation and Characterization of Glass Ionomer Cements with Added Carboxymethyl Chitosan |
|
| Xiaozhen Bao, Fang Liu, Jingwei He | | Journal of Macromolecular Science, Part B. 2020; 59(6): 345 | | [Pubmed] | [DOI] | | | 14 |
Biopolymers for hydrogels in cosmetics: review |
|
| Stanislaw Mitura, Alina Sionkowska, Amit Jaiswal | | Journal of Materials Science: Materials in Medicine. 2020; 31(6) | | [Pubmed] | [DOI] | | | 15 |
Applications of Natural, Semi-Synthetic, and Synthetic Polymers in Cosmetic Formulations |
|
| Thais F. R. Alves, Margreet Morsink, Fernando Batain, Marco V. Chaud, Taline Almeida, Dayane A. Fernandes, Classius F. da Silva, Eliana B. Souto, Patricia Severino | | Cosmetics. 2020; 7(4): 75 | | [Pubmed] | [DOI] | | | 16 |
Nanoparticles as Anti-Microbial, Anti-Inflammatory, and Remineralizing Agents in Oral Care Cosmetics: A Review of the Current Situation |
|
| Florence Carrouel, Stephane Viennot, Livia Ottolenghi, Cedric Gaillard, Denis Bourgeois | | Nanomaterials. 2020; 10(1): 140 | | [Pubmed] | [DOI] | | | 17 |
Fluoride varnish containing chitosan demonstrated sustained fluoride release |
|
| Woradej PICHAIAUKRIT,Niyom THAMRONGANANSKUL,Krisana SIRALERTMUKUL,Somporn SWASDISON | | Dental Materials Journal. 2019; | | [Pubmed] | [DOI] | | | 18 |
Fluoride varnish containing chitosan demonstrated sustained fluoride release |
|
| Woradej PICHAIAUKRIT,Niyom THAMRONGANANSKUL,Krisana SIRALERTMUKUL,Somporn SWASDISON | | Dental Materials Journal. 2019; | | [Pubmed] | [DOI] | | | 19 |
Chitosan Use in Dentistry: A Systematic Review of Recent Clinical Studies |
|
| Masoud Cicciù,Masoud Fiorillo,Masoud Cervino | | Marine Drugs. 2019; 17(7): 417 | | [Pubmed] | [DOI] | | | 20 |
Chitosan Use in Dentistry: A Systematic Review of Recent Clinical Studies |
|
| Masoud Cicciù,Masoud Fiorillo,Masoud Cervino | | Marine Drugs. 2019; 17(7): 417 | | [Pubmed] | [DOI] | | | 21 |
Stevia rebaudiana
Bertoni bioactive effects: From in vivo to clinical trials towards future therapeutic approaches |
|
| Bahare Salehi,Maria Dolores López,Sara Martínez-López,Montserrat Victoriano,Javad Sharifi-Rad,Miquel Martorell,Célia F. Rodrigues,Natália Martins | | Phytotherapy Research. 2019; 33(11): 2904 | | [Pubmed] | [DOI] | | | 22 |
Stevia rebaudiana
Bertoni bioactive effects: From in vivo to clinical trials towards future therapeutic approaches |
|
| Bahare Salehi,Maria Dolores López,Sara Martínez-López,Montserrat Victoriano,Javad Sharifi-Rad,Miquel Martorell,Célia F. Rodrigues,Natália Martins | | Phytotherapy Research. 2019; 33(11): 2904 | | [Pubmed] | [DOI] | | | 23 |
Application of biosurfactants and chitosan in toothpaste formulation |
|
| Ana Helena M. Resende,Juliana M. Farias,Dhanilo D.B. Silva,Raquel D. Rufino,Juliana M. Luna,Thayza Christina M. Stamford,Leonie A. Sarubbo | | Colloids and Surfaces B: Biointerfaces. 2019; 181: 77 | | [Pubmed] | [DOI] | | | 24 |
Application of biosurfactants and chitosan in toothpaste formulation |
|
| Ana Helena M. Resende,Juliana M. Farias,Dhanilo D.B. Silva,Raquel D. Rufino,Juliana M. Luna,Thayza Christina M. Stamford,Leonie A. Sarubbo | | Colloids and Surfaces B: Biointerfaces. 2019; 181: 77 | | [Pubmed] | [DOI] | | | 25 |
Chitosan in Biomedical Engineering: A Critical Review |
|
| Shabnam Mohebbi,Mojtaba Nasiri Nezhad,Payam Zarrintaj,Seyed Hassan Jafari,Saman Seyed Gholizadeh,Mohammad Reza Saeb,Masoud Mozafari | | Current Stem Cell Research & Therapy. 2019; 14(2): 93 | | [Pubmed] | [DOI] | | | 26 |
Chitosan in Biomedical Engineering: A Critical Review |
|
| Shabnam Mohebbi,Mojtaba Nasiri Nezhad,Payam Zarrintaj,Seyed Hassan Jafari,Saman Seyed Gholizadeh,Mohammad Reza Saeb,Masoud Mozafari | | Current Stem Cell Research & Therapy. 2019; 14(2): 93 | | [Pubmed] | [DOI] | | | 27 |
Chitosan/Fluoride Nanoparticles for Preventing Dental Caries |
|
| Niousha Ebrahimi,Ali Asghar Soleimani,Jamal Rashidiani,Beheshteh Malekafzali,Fatemeh Abedini,Hossein Hosseinkhani | | Current Dentistry. 2019; 1(1): 61 | | [Pubmed] | [DOI] | | | 28 |
Chitosan/Fluoride Nanoparticles for Preventing Dental Caries |
|
| Niousha Ebrahimi,Ali Asghar Soleimani,Jamal Rashidiani,Beheshteh Malekafzali,Fatemeh Abedini,Hossein Hosseinkhani | | Current Dentistry. 2019; 1(1): 61 | | [Pubmed] | [DOI] | | | 29 |
Chitosan as biomaterial in drug delivery and tissue engineering |
|
| Saad M. Ahsan,Mathai Thomas,Kranthi K. Reddy,Sujata Gopal Sooraparaju,Amit Asthana,Ira Bhatnagar | | International Journal of Biological Macromolecules. 2018; 110: 97 | | [Pubmed] | [DOI] | | | 30 |
Chitosan as biomaterial in drug delivery and tissue engineering |
|
| Saad M. Ahsan,Mathai Thomas,Kranthi K. Reddy,Sujata Gopal Sooraparaju,Amit Asthana,Ira Bhatnagar | | International Journal of Biological Macromolecules. 2018; 110: 97 | | [Pubmed] | [DOI] | | | 31 |
Antibacterial and antifungal effects of chitosan nanoparticles on tissue conditioners of complete dentures |
|
| Seyed Amin Mousavi,Reza Ghotaslou,Shirafkan Kordi,Azin Khoramdel,Ali Aeenfar,Sona Talaei Kahjough,Abolfazl Akbarzadeh | | International Journal of Biological Macromolecules. 2018; 118: 881 | | [Pubmed] | [DOI] | | | 32 |
Antibacterial and antifungal effects of chitosan nanoparticles on tissue conditioners of complete dentures |
|
| Seyed Amin Mousavi,Reza Ghotaslou,Shirafkan Kordi,Azin Khoramdel,Ali Aeenfar,Sona Talaei Kahjough,Abolfazl Akbarzadeh | | International Journal of Biological Macromolecules. 2018; 118: 881 | | [Pubmed] | [DOI] | | | 33 |
Cosmetics and Cosmeceutical Applications of Chitin, Chitosan and Their Derivatives |
|
| Inmaculada Aranaz,Niuris Acosta,Concepción Civera,Begoña Elorza,Javier Mingo,Carolina Castro,María Gandía,Angeles Heras Caballero | | Polymers. 2018; 10(2): 213 | | [Pubmed] | [DOI] | | | 34 |
Cosmetics and Cosmeceutical Applications of Chitin, Chitosan and Their Derivatives |
|
| Inmaculada Aranaz,Niuris Acosta,Concepción Civera,Begoña Elorza,Javier Mingo,Carolina Castro,María Gandía,Angeles Heras Caballero | | Polymers. 2018; 10(2): 213 | | [Pubmed] | [DOI] | | | 35 |
Clinicomicrobiological Evaluation of 2% Chitosan
Mouthwashes on Dental Plaque |
|
| Sheetal P Mhaske, Divya Iska, Rajesh Ambiti, Umang Jagga, Uttam Paul, Shruthi M Shanmukappa | | The Journal of Contemporary Dental Practice. 2018; 19(1): 94 | | [Pubmed] | [DOI] | | | 36 |
Nanochitosan modified glass ionomer cement with enhanced mechanical properties and fluoride release |
|
| R. Senthil Kumar,N. Ravikumar,S. Kavitha,S. Mahalaxmi,R. Jayasree,T.S. Sampath Kumar,M. Haneesh | | International Journal of Biological Macromolecules. 2017; 104: 1860 | | [Pubmed] | [DOI] | | | 37 |
Nanochitosan modified glass ionomer cement with enhanced mechanical properties and fluoride release |
|
| R. Senthil Kumar,N. Ravikumar,S. Kavitha,S. Mahalaxmi,R. Jayasree,T.S. Sampath Kumar,M. Haneesh | | International Journal of Biological Macromolecules. 2017; 104: 1860 | | [Pubmed] | [DOI] | | | 38 |
Chitosan-Properties and Applications in Dentistry |
|
| Kmiec M | | Advances in Tissue Engineering & Regenerative Medicine: Open Access. 2017; 2(4) | | [Pubmed] | [DOI] | | | 39 |
Chitosan-Properties and Applications in Dentistry |
|
| Kmiec M | | Advances in Tissue Engineering & Regenerative Medicine: Open Access. 2017; 2(4) | | [Pubmed] | [DOI] | | | 40 |
Dentifrice Containing Extract of Rosmarinus officinalis Linn.: An Antimicrobial Evaluation |
|
| Marcela Agne Alves Valones,Jane Sheila Higino,Paulo Roberto Eleutério Souza,Sérgio Crovella,Arnaldo de França Caldas Júnior,Alessandra de Albuquerque Tavares Carvalho | | Brazilian Dental Journal. 2016; 27(5): 497 | | [Pubmed] | [DOI] | | | 41 |
Dentifrice Containing Extract of Rosmarinus officinalis Linn.: An Antimicrobial Evaluation |
|
| Marcela Agne Alves Valones,Jane Sheila Higino,Paulo Roberto Eleutério Souza,Sérgio Crovella,Arnaldo de França Caldas Júnior,Alessandra de Albuquerque Tavares Carvalho | | Brazilian Dental Journal. 2016; 27(5): 497 | | [Pubmed] | [DOI] | | | 42 |
Is Stevia rebaudiana Bertoni a Non Cariogenic Sweetener? A Review |
|
| Gianmaria Ferrazzano,Tiziana Cantile,Brunella Alcidi,Marco Coda,Aniello Ingenito,Armando Zarrelli,Giovanni Di Fabio,Antonino Pollio | | Molecules. 2015; 21(1): 38 | | [Pubmed] | [DOI] | | | 43 |
Is Stevia rebaudiana Bertoni a Non Cariogenic Sweetener? A Review |
|
| Gianmaria Ferrazzano,Tiziana Cantile,Brunella Alcidi,Marco Coda,Aniello Ingenito,Armando Zarrelli,Giovanni Di Fabio,Antonino Pollio | | Molecules. 2015; 21(1): 38 | | [Pubmed] | [DOI] | | | 44 |
Chitosan mouthwash: Toxicity and in vivo validation |
|
| E.M. Costa,S. Silva,S. M.R.Costa,M. Pereir,D.A. Campos,J. Odila,A.R. Madureira,A. Cardelle-Cobas,F.K. Tavaria,A.S. Rodrigues,M.M. Pintado | | Carbohydrate Polymers. 2014; | | [Pubmed] | [DOI] | | | 45 |
Chitosan as a dental biomaterial: State of the art [A quitosana como biomaterial odontológico: Estado da arte] |
|
| Tavaria, F.K. and Costa, E.M. and Pina-Vaz, I. and Carvalho, M.F. and Pintado, M.M. | | Revista Brasileira de Engenharia Biomedica. 2013; 29(1): 110-120 | | [Pubmed] | | | 46 |
Aloe vera herbal dentifrices for plaque and gingivitis control: a systematic review |
|
| K Dhingra | | Oral Diseases. 2013; : n/a | | [Pubmed] | [DOI] | | | 47 |
RETRACTED: Synthesis and characterization of chitosan and grape polyphenols stabilized palladium nanoparticles and their antibacterial activity |
|
| Kanchana Amarnath,Jayanthi Kumar,Tejesh Reddy,Vakka Mahesh,Senniyanallur Rathakrishnan Ayyappan,Jayshree Nellore | | Colloids and Surfaces B: Biointerfaces. 2012; 92: 254 | | [Pubmed] | [DOI] | | | 48 |
Assessment of Dentifrices Against Candida Biofilm |
|
| Nivedita Singh,Akhansha Nayyar,G. Bhattacharjee,A. K. Singh,Vikas Pruthi | | Applied Biochemistry and Biotechnology. 2012; 167(6): 1688 | | [Pubmed] | [DOI] | | | 49 |
Assessment of dentifrices against Candida biofilm |
|
| Singh, N. and Nayyar, A. and Bhattacharjee, G. and Singh, A.K. and Pruthi, V. | | Applied Biochemistry and Biotechnology. 2012; 167(6): 1688-1698 | | [Pubmed] | | | 50 |
Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive |
|
| Szczepanska, J., Pawlowska, E., Synowiec, E., Czarny, P., Rekas, M., Blasiak, J., Szaflik, J.P. | | Medical Science Monitor. 2011; 17(8): 201-208 | | [Pubmed] | | | 51 |
Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive |
|
| Joanna Szczepanska,Elzbieta Pawlowska,Ewelina Synowiec,Piotr Czarny,Marek Rekas,Janusz Blasiak,Jacek Pawel Szaflik | | Medical Science Monitor. 2011; 17(8): BR201 | | [Pubmed] | [DOI] | |
|
|
|
|
|
|
|
|
|
|
|
|
| Article Access Statistics | | | Viewed | 21166 | | | Printed | 857 | | | Emailed | 12 | | | PDF Downloaded | 736 | | | Comments | [Add] | | | Cited by others | 51 | | |
|
|
Users online:
