Indian Journal of Dental Research

ORIGINAL RESEARCH
Year
: 2019  |  Volume : 30  |  Issue : 2  |  Page : 273--276

The difference in biofilm molecular weight in Streptococcus mutans and Aggregatibacter actinomycetemcomitans induced by sucrose and soy protein (glycine soja)


Indah Listiana Kriswandini, Markus Budi Rahardjo, Hendrik Setia Budi, Risma Amalia 
 Oral Biology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Correspondence Address:
Dr. Indah Listiana Kriswandini
Jl. Prof. Dr. Moestopo No. 47 Surabaya
Indonesia

Context: Biofilms consist of microbial cells and extracellular polymeric substance (EPS). Streptococcus mutans and Aggregatibacter actinomycetemcomtans are bacteria that can form biofilms and generate EPS. Biofilm formation can be induced by specific substances such as sucrose and protein. Aims: To identify the molecular weight that determines biofilm protein profile expression of S. mutans and A. actinomycetemcomitans induced by sucrose (carbohydrate) and soy protein (glycine soja). Settings and Design: Experimental laboratory study. Materials and Methods: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the molecular weight. Statistical Analysis Used: Nil. Results: The results of analysis of protein SDS-PAGE showed the presence of 28 protein bands on A. actinomycetemcomitans biofilm in the media trypticase soy broth (TSB), 20 protein bands on biofilms of S. mutans in the media TSB, 29 protein bands on biofilm A. actinomycetemcomitans in the media brain heart infusion (BHI) + sucrose 2%, and 13 protein bands on biofilms of S. mutans in the media BHI + sucrose 2%. Conclusion: There are differences in biofilm protein profile expression that determine the molecular weight of S. mutans biofilm and A. actinomycetemcomitans induced by sucrose (carbohydrate) and soy protein (glycine soja).


How to cite this article:
Kriswandini IL, Rahardjo MB, Budi HS, Amalia R. The difference in biofilm molecular weight in Streptococcus mutans and Aggregatibacter actinomycetemcomitans induced by sucrose and soy protein (glycine soja).Indian J Dent Res 2019;30:273-276


How to cite this URL:
Kriswandini IL, Rahardjo MB, Budi HS, Amalia R. The difference in biofilm molecular weight in Streptococcus mutans and Aggregatibacter actinomycetemcomitans induced by sucrose and soy protein (glycine soja). Indian J Dent Res [serial online] 2019 [cited 2020 Apr 10 ];30:273-276
Available from: http://www.ijdr.in/article.asp?issn=0970-9290;year=2019;volume=30;issue=2;spage=273;epage=276;aulast=Kriswandini;type=0