| Abstract|| |
Background: The aim of the study is to comparatively evaluate the levels of cathepsin K (CSTK) in gingival crevicular fluid (GCF) among smoking and nonsmoking patients with chronic periodontitis (CP). Materials and Methods: A total of 160 systemically healthy male patients were included in the study. Based on probing pocket depth, clinical attachment level, plaque index, and modified sulcular bleeding index, the patients were allotted into four groups: Group I - with forty subjects who were smokers with healthy periodontium, Group II - with forty nonsmoking subjects with healthy periodontium, Group III - forty patients who were smokers with CP, and Group IV - with forty nonsmoking CP patients. Those who claimed to have never smoked were recruited into the nonsmoker group, whereas subjects who reported smoking ≥10 cigarettes per day for more than 5 years were recruited into the smoker group. The GCF samples were collected using microcapillary pipettes and analyzed for levels of CSTK using enzyme-linked immunosorbent assay. Results: The GCF concentration of CSTK was expressed in pg/μl. The mean CSTK levels in the groups were Group I - 0.158 ± 0.043 pg/μl, Group II - 0.145 ± 0.026 pg/μl, Group III - 15.768 ± 12.40 pg/μl, and for Group IV - 11.59 ± 12.15 pg/μl, respectively. The levels of CSTK were statistically higher in Group III when compared with Group IV (P = 0.037) (P < 0.05). Conclusion: CSTK levels were significantly increased in smokers with CP than nonsmokers, suggesting a positive influence of smoking on CSTK which could possibly play a role in the increased susceptibility for osteoclastic bone destruction in smoker subjects.
Keywords: Cathepsin K, gingival crevicular fluid, periodontitis, smoking
|How to cite this article:|
Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res 2018;29:588-93
|How to cite this URL:|
Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res [serial online] 2018 [cited 2020 Feb 21];29:588-93. Available from: http://www.ijdr.in/text.asp?2018/29/5/588/244955
| Introduction|| |
Our understanding of periodontal diseases has evolved over the years and has transformed from periodontitis being considered an almost ubiquitous condition, in which the role of plaque as the sole etiological factor was unquestioned to the current understanding of the pivotal role of host immune inflammatory response together with considerable knowledge on the influence of various individual risk factors suggesting that periodontitis is a multifactorial polymicrobial disease.
Cigarette smoking is the strongest of the modifiable risk factors for periodontitis secondary to bacterial plaque. Evidence from various studies has shown that adult smokers are about two to four times more likely to have periodontitis than nonsmokers.,, So far, the effects of smoking on subgingival microflora,,, gingival vasculature,,, neutrophils, serum IgG, and circulating levels of cytokines, have been reported. Although various studies have attempted to explain the mechanism of action of smoking in the pathogenesis of periodontitis,, the molecular basis of how smoking contributes to alveolar bone loss is still poorly understood.
Substances produced by the subgingival bacterial flora and the tissue during inflammation and immune reactions may affect bone turnover by causing the differentiation and stimulation of osteoclasts or by inhibiting bone formation by osteoblasts. Osteoclasts are cells that play pivotal roles in bone morphogenesis, remodeling, and resorption, differentiate from the hematopoietic myeloid precursors of macrophage/monocyte lineage.,,,,
Proteolytic processes are known to be critical in osteoclastic bone resorption. Degradation of bone matrix proteins is initiated by acidic lysosomal proteinases secreted from osteoclasts into the bone resorption lacunae and then is continued and completed in the intracellular endosomal/lysosomal system. So far, a variety of lysosomal proteinases have been identified in both the lacuna and the lysosome-like organelles in actively resorbing osteoclasts.,,, Among the various lysosomal proteinases, recent data, however, strongly implicate cathepsin K (CSTK) as the predominant effector in osteoclastic bone resorption.
CSTK expression is required for normal skeletal development., Nonsense, missense, and stop codon mutations in the CSTK gene have been identified in humans with pycnodysostosis, which is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.,,
Literature reports the involvement of CSTK in various disorders associated with bone resorption such as osteoporosis, Paget's disease, diffuse sclerosing osteomyelitis of mandible, and the critical role of CSTK in the pathogenesis of rheumatoid arthritis., Abnormally high CSTK production is reported in ankylosing spondylitis and atherosclerosis. Recent studies have reported elevated levels of CSTK in gingival crevicular fluid (GCF) of patients with periodontitis and peri-implantitis.
Apart from an in vitro study by Tanaka et al., 2013, till date to the best of our knowledge, no human studies have documented the influence of smoking as an environmental factor to regulate the levels of CSTK. Hence, the present study was designed to comparatively evaluate the influence of an environmental factor - smoking on the levels of CSTK in GCF among chronic periodontitis (CP) patients.
| Materials and Methods|| |
Patients seeking dental treatment in the department of periodontics were recruited in this study. This study was approved by the Institutional Review Board. All the patients who participated were informed of the nature of the study and those willing to participate, duly signed the consent form.
Eighty systemically healthy male subjects with CP (forty smokers and forty nonsmokers) and eighty male systemically, periodontally healthy subjects (forty smokers and forty nonsmokers) were recruited in the study. The healthy group consisted of patients with ≥90% of the measured sites exhibiting probing pocket depth (PPD) <3 mm and clinical attachment level (CAL) = 0 mm, no bleeding on probing, and no radiographic sign of alveolar bone loss (i.e. a distance of <3 mm between the cementoenamel junction and bone crest at >95% of the proximal tooth sites). Group I had forty smokers with healthy periodontium and Group II had 40 nonsmokers with healthy periodontium.
CP patients were diagnosed in accordance with the clinical criteria stated in the consensus report of the World Workshop in Periodontitis. Those who claimed to have never smoked were recruited into the nonsmoker group, whereas subjects who reported smoking ≥10 cigarettes per day for more than 5 years were recruited into the smoker group. Patients who smoked ≥10 cigarettes per day for <5 years and those who smoked <10 cigarettes per day for more than 5 years were excluded to better differentiate smokers from nonsmokers. Group III had forty smokers with CP and Group IV had forty nonsmokers with CP.
Patients with aggressive periodontitis, diabetes, hypertension, gross oral pathology, heart disease, rheumatoid or osteoarthritis, tumors, or any other systemic disease which can alter the course of periodontal disease, on any medication such as phenytoin, cyclosporine, calcium channel blockers, bisphosphonates, vitamin D, or calcium supplements and who had taken antibiotics, anti-inflammatory drugs or received periodontal therapy in the preceding 6 months, and females were excluded from the study.
All patients had a clinical periodontal examination which included the measurement of PPD and CAL at six sites around each tooth with a manual probe University of North Carolina-15. Plaque index (PI) (Silness and Loe, 1964) and modified sulcular bleeding index (mSBI) (A Mombelli, 1987) were also recorded. All measurements were performed by a single calibrated examiner. Only one site with deepest PD per patient was selected as the sampling site in all the groups.
After isolating the tooth with a cotton roll, supragingival plaque was removed with a curette without touching the marginal gingiva. The crevicular site was then dried gently with an air syringe. GCF was collected by placing a microcapillary pipette at the entrance of the gingival sulcus, gently touching the gingival margin. From each group, a standardized volume of 2–3 μl was collected using the calibration on white color-coded 1–5 μl calibrated volumetric microcapillary pipette (Sigma-Aldrich, St. Louis, MO, USA). Each sample collection was allotted a maximum of 10 min and the sites which did not express GCF within the allotted time were excluded. This was carried be out to ensure atraumatism. The micropipettes that were suspected to be contaminated with blood or saliva were excluded. The collected GCF samples were transferred to airtight plastic vials and stored at −80°C until assayed. For analysis, 200 μl phosphate-buffered saline (pH 7.2) was used to re-elute the samples at the time of performing the assay.
The enzyme-linked immunosorbent assay kit for quantification of human CSTK levels in GCF was purchased from USCN life sciences, Inc., USA, and the assay was performed according to the manufacturer's recommendations. The detection limit of the assay as reported by the manufacturer is from 0.156 ng/mL to 10 ng/mL. Data were then calculated and obtained by methods of interpolation of a predetermined standard curve. As the samples have been diluted, the concentration read from the standard curve has been multiplied by the dilution factor. Values of total amounts are expressed as pg/μL. The concentration of CSTK in the samples is then determined by comparing the optical density of the samples to the standard curve.
Clinical parameters as well as the total amount and concentration of CSTK in healthy and CP groups were expressed as mean ± standard deviation. A Levene test for equality of variance showed that the CSTK values in Group III and IV were not normally distributed. Hence, nonparametric statistical analysis was performed when necessary.
Student t-test (two-tailed, independent) has been used to compare the clinical parameters between Group I/II and Group III/IV. Student's t-test was used to compare GCF concentrations of RANKL and CSTK in Group I and II. Mann–Whitney U-test has been used to compare the values of cytokine CSTK between smoking and nonsmoking healthy and CP patients.
The correlation between CSTK with the clinical parameters in Group I and II was done using Pearson correlation analysis. The correlation between CSTK with clinical parameters in Group III and IV was done using Kendall Tau-b correlation analysis. P < 0.05 is considered statistically significant for all the statistical analyses. All data were analyzed using a software program (SPSS Version 15, SPSS Inc., Chicago, IL, USA).
| Results|| |
Clinical findings of study groups
The descriptive statistics of the study population showing mean and standard deviation for age, PPD, CAL, PLI, and mSBI and pack years are represented in [Table 1] and [Table 2].
Comparison of clinical parameters among the study groups
When clinical parameters were compared between Group III and IV, PPD and CAL were significantly higher in Group III than Group IV with P = 0.003 and 0.001, respectively. The gingival index was statistically higher in nonsmokers than smokers with CP (P = 0.014). There was statistically no significant difference between Group III and IV in plaque index [Table 3].
Analysis of cathepsin concentration in gingival crevicular fluid
The mean concentration of CSTK of all the groups is shown in [Table 4].
|Table 4: Mean concentration cathepsin K in gingival crevicular fluid of all the study groups|
Click here to view
CSTK levels were detected only in eight patients in each of Group I and II. CSTK levels were detected in all patients in Group III and IV.
When comparing the CSTK concentration among Group I and II patients, there was no significant difference between the groups (P = 0.496) (P < 0.05). There was a significantly higher concentration of CSTK levels in smokers when compared with nonsmoking CP patients (Group III and IV) (P = 0.037) (P < 0.05) [Table 5]. The CSTK levels were significantly higher in Group III (smokers CP) when compared with Group I (healthy CP) (P = 0.000) (P < 0.01), and similarly, the CSTK levels were significantly higher in Group IV (nonsmokers CP) when compared with Group II (nonsmoking healthy) (P = 0.000) (P < 0.01) [Table 5].
|Table 5: Comparison cathepsin K levels in gingival crevicular fluid between Groups|
Click here to view
| Discussion|| |
Smoking influences angiogenesis,, adhesion molecule profiles and leukocyte recruitment,, multiple aspects of inflammatory response,,, and homeostasis and healing potential of the periodontal connective tissue., Although smokers have been reported to be more susceptible to advanced and aggressive forms of periodontal disease than nonsmokers,,,, the exact molecular mechanisms by which smoking exerts detrimental effects on the periodontal tissue remains conflicting.
In the present study, females were excluded intentionally as it would be difficult to recruit females who admit that they smoke. The other reasons for excluding them were to avoid potential hormonal influences on the periodontium.,
In the present study, CSTK levels in the GCF showed a significant increase (P = 0.000) (P < 0.01) in the nonsmokers CP (Group IV) group when compared with the nonsmoker healthy subjects (Group II). This present finding is in accordance with the results of Mogi and Otogoto et al. and Garg et al. reporting elevated concentration of CSTK in the GCF of CP patients when compared with healthy individuals. A decrease in GCF levels of CSTK following nonsurgical periodontal therapy was shown by Garg et al. in patients with periodontitis which further supports the role of CSTK in osteoclastic bone resorption in periodontal disease.
To the best of our knowledge, till date, this is the first study to evaluate the influence of smoking on CSTK levels in the GCF of CP patients. There was a significantly higher concentration of CSTK levels in smokers when compared with nonsmoking CP patients (Group III and IV) (P = 0.037). This finding suggests that smoking has a positive influence on CSTK levels in CP patients; however, the mechanism remains unclear.
Studies done by Cesar Neto et al. and Boström et al. have suggested that smoking favors osteoclastogenesis by an increased production of pro-inflammatory cytokines such as IL-6 and tumor necrosis factor-alpha (TNF-α). An in vitro study by Kudo et al. demonstrated that cytokines, TNF-α, and IL-1α directly induced osteoclastogenesis in peripheral blood mononuclear cell which were positive for tartrate-resistant acid phosphatase and CSTK by a process which is distinct from the RANK/RANKL signaling pathway. In addition, Teng et al. showed that the inflammatory mediators such as IL-1 β, TNF-α, IL-6, IL-11, IL-17, and PGE2 can regulate osteoclastic activity through RANK-independent pathways also. Although the RANKL-dependent pathway is crucial for osteoclastogenesis, the presence of increased levels of potent osteogenic inflammatory mediators may drive RANKL independent pathway in various chronic pathological bone resorptive conditions. Such RANKL independent-cytokine dependent pathway could have influenced the CSTK levels in the present study. However, this hypothesis needs to be carefully elucidated by further studies in the future.
In contrary to the present study finding, an in vitro study by Tanaka et al. on the direct effects of nicotine on RAW264.7 cells suggested that nicotine had a suppressive effect on the expression of CSTK in activated osteoclasts. However, this in vitro model which has shown the effects of nicotine on bone marrow osteoclastic cell lines may not reflect or recreate the complex micro/macroenvironment of the human periodontium and the multifactorial polymicrobial nature of periodontal pathogenesis.
No significant correlations between CSTK levels and any of the clinical parameters were obtained in the nonsmokers CP (Group IV) whereas the study by Garg et al. showed a negative correlation between the clinical parameters such as PD and CAL with CSTK levels and the negative correlation was attributed to the consumption of CSTK in degradation of Type I collagen at its noncollagenous termini and release of cross-linked N and C telopeptides.
In the present study, the oral hygiene status as depicted by plaque scores was almost similar between the smoking and nonsmoking CP group and this finding is in agreement with the other previous studies.,,,, Contradicting the present study finding, other studies have shown higher plaque levels in smokers.,,,,, The gingival index was statistically higher in nonsmokers than smokers with CP (P = 0.014) (P < 0.05) which is in agreement with the earlier studies.,,
| Conclusion|| |
Although smoking has shown a positive influence on CSTK levels, further studies are needed to investigate peptide and mRNA expression levels of CSTK in gingival tissues at various stages of periodontitis, also following conventional and regenerative periodontal therapy in smokers with CP to provide deepest understanding on the role of CSTK in periodontal pathogenesis and if CSTK inhibitors could be used as potential host modulating agents.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Ainamo J, Ainamo A. Risk assessment of recurrence of disease during supportive periodontal care. Epidemiological considerations. J Clin Periodontol 1996;23:232-9.
Johnson GK, Hill M. Cigarette smoking and the periodontal patient. J Periodontol 2004;75:196-209.
Johnston JD. Smokers have less dense bones and fewer teeth. J R Soc Health 1994;114:265-9.
Hafajee AD, Socransky SS. Relationship of cigarette smoking to the subgingival microbiota. J Clin Periodontol 2001;28:377-88.
Umeda M, Chen C, Bakker, I, Contreas A, Morrison JL, Slots J. Risk indicators for harboring periodontal pathogens. J Periodontol 1998;69:1111-8.
Zambon JJ, Grossi SG, Machtei EE, Ho AW, Dunford R, Genco RJ, et al.
Cigarette smoking increases the risk for subgingival infection with periodontal pathogens. J Periodontol 1996;67:1050-4.
Bergström J, Persson L, Preber H. Influence of cigarette smoking on vascular reaction during experimental gingivitis. Scand J Dent Res 1988;96:34-9.
Clarke NG, Shephard BC, Hirsch RS. The effects of intra-arterial epinephrine and nicotine on gingival circulation. Oral Surg Oral Med Oral Pathol 1981;52:577-82.
Rezavandi, K, Palmer RM, Odell EW, Scott DA, Wilson RF. Expression of ICAM-1 and E-selectin in gingival tissues of smokers and non-smokers with periodontitis. J Oral Pathol Med 2002; 31:59-64.
Gustafsson A, Asman B, Bergström K. Cigarette smoking as an aggravating factor in inflammatory tissue-destructive diseases. Increase in tumor necrosis factor-alpha priming of peripheral neutrophils measured as generation of oxygen radicals. Int J Clin Lab Res 2000;30:187-90.
Quinn SM, Zhang JB, Gunsolley JC, Schenkein HA, Tew JG. The influence of smoking and race on adult periodontitis and serum IgG2 levels. J Periodontol 1998;69:171-7.
Boström L, Linder LE, Bergström J. Clinical expression of TNF-alpha in smoking-associated periodontal disease. J Clin Periodontol 1998;25:767-73.
Petropoulos G, McKay IJ, Hughes FJ. The association between neutrophil numbers and interleukin-1alpha concentrations in gingival crevicular fluid of smokers and non-smokers with periodontal disease. J Clin Periodontol 2004;31:390-5.
Bergström J, Eliasson S, Dock J. A 10-year prospective study of tobacco smoking and periodontal health. J Periodontol 2000;71:1338-47.
Jansson L, Lavstedt S. Influence of smoking on marginal bone loss and tooth loss – a prospective study over 20 years. J Clin Periodontol 2002;29:750-6.
Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ, et al.
Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev 1999;20:345-57.
Karsenty G, Wagner EF. Reaching a genetic and molecular understanding of skeletal development. Dev Cell 2002;2:389-406.
Manolagas SC. Birth and death of bone cells: Basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev 2000;21:115-37.
Lee ZH, Kim HH. Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts. Biochem Biophys Res Commun 2003;305:211-4.
Takahashi N, Udagawa N, Tanaka S, Murakami H, Owan I, Tamura T, et al.
Postmitotic osteoclast precursors are mononuclear cells which express macrophage-associated phenotypes. Dev Biol 1994;163:212-21.
Goto T, Kiyoshima T, Moroi R, Tsukuba T, Nishimura Y, Himeno M, et al.
Localization of cathepsins B, D, and L in the rat osteoclast by immuno-light and -electron microscopy. Histochemistry 1994;101:33-40.
Sasaki T, Ueno-Matsuda E. Cysteine-proteinase localization in osteoclasts: An immunocytochemical study. Cell Tissue Res 1993;271:177-9.
Yoshimine Y, Tsukuba T, Isobe R, Sumi M, Akamine A, Maeda K, et al.
Specific immunocytochemical localization of cathepsin E at the ruffled border membrane of active osteoclasts. Cell Tissue Res 1995;281:85-91.
Drake FH, Dodds RA, James IE, Connor JR, Debouck C, Richardson S, et al.
Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts. J Biol Chem 1996;271:12511-6.
Troen BR. The role of cathepsin K in normal bone resorption. Drug News Perspect 2004;17:19-28.
Matsumoto M, Kogawa M, Wada S, Takayanagi H, Tsujimoto M, Katayama S, et al.
Essential role of p38 mitogen-activated protein kinase in cathepsin K gene expression during osteoclastogenesis through association of NFATc1 and PU.1. J Biol Chem 2004;279:45969-79.
Troen BR. Cathepsin genes: Structure, expression and regulation. In: Ouali A, Demeyer DI, Smulders FJ, editors. Expression of Tissue Proteinases and Regulation of Protein Degradation as Related to Meat Quality. The Netherlands: Utrecht: Ecceamst. 1995. p. 29-46.
Gelb BD, Shi GP, Chapman HA, Desnick RJ. Pycnodysostosis, a lysosomal disease caused by cathepsin K deficiency. Science 1996;273:1236-8.
Johnson MR, Polymeropoulos MH, Vos HL, Ortiz de Luna RI, Francomano CA. A nonsense mutation in the cathepsin K gene observed in a family with pycnodysostosis. Genome Res 1996;6:1050-5.
Hou WS, Brömme D, Zhao Y, Mehler E, Dushey C, Weinstein H, et al.
Characterization of novel cathepsin K mutations in the pro and mature polypeptide regions causing pycnodysostosis. J Clin Invest 1999;103:731-8.
Meier C, Meinhardt U, Greenfield JR, De Winter J, Nguyen TV, Dunstan CR, et al.
Serum cathepsin K concentrations reflect osteoclastic activity in women with postmenopausal osteoporosis and patients with Paget's disease. Clin Lab 2006;52:1-10.
Montonen M, Li TF, Lukinmaa PL, Sakai E, Hukkanen M, Sukura A, et al.
RANKL and cathepsin K in diffuse sclerosing osteomyelitis of the mandible. J Oral Pathol Med 2006;35:620-5.
Skoumal M, Haberhauer G, Kolarz G, Hawa G, Woloszczuk W, Klingler A. Serum Cathepsin K levels of patients with long lasting rheumatoid arthritis: Correlation with radiological destruction. Arthritis Res Ther 2005;7:R65-70.
Wendling D, Cedoz JP, Racadot E. Serum levels of MMP-3 and cathepsin K in patients with ankylosing spondylitis: Effect of TNFalpha antagonist therapy. Joint Bone Spine 2008;75:559-62.
Lutgens E, Lutgens SP, Faber BC, Heeneman S, Gijbels MM, de Winther MP, et al.
Disruption of the cathepsin K gene reduces atherosclerosis progression and induces plaque fibrosis but accelerates macrophage foam cell formation. Circulation 2006;113:98-107.
Strbac GD, Monov G, Cei S, Kandler B, Watzek G, Gruber R, et al.
Cathepsin K levels in the crevicular fluid of dental implants: A pilot study. J Clin Periodontol 2006;33:302-8.
Tanaka H, Tanabe N, Kawato T, Nakai K, Kariya T, Matsumoto S, et al.
Nicotine affects bone resorption and suppresses the expression of cathepsin K, MMP-9 and vacuolar-type H(+)-ATPase d2 and actin organization in osteoclasts. PLoS One 2013;8:e59402.
Ozçaka O, Nalbantsoy A, Köse T, Buduneli N. Plasma osteoprotegerin levels are decreased in smoker chronic periodontitis patients. Aust Dent J 2010;55:405-10.
Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4:1-6.
Buduneli N, Buduneli E, Kardeşler L, Lappin D, Kinane DF. Plasminogen activator system in smokers and non-smokers with and without periodontal disease. J Clin Periodontol 2005;32:417-24.
Scott DA, Palmer RM. The influence of tobacco smoking on adhesion molecule profiles. Tob Induc Dis 2002;1:7-25.
Seow WK, Thong YH, Nelson RD, MacFarlane GD, Herzberg MC. Nicotine-induced release of elastase and eicosanoids by human neutrophils. Inflammation 1994;18:119-27.
Barbour SE, Nakashima K, Zhang JB, Tangada S, Hahn CL, Schenkein HA, et al.
Tobacco and smoking: Environmental factors that modify the host response (immune system) and have an impact on periodontal health. Crit Rev Oral Biol Med 1997;8:437-60.
van Eeden SF, Hogg JC. The response of human bone marrow to chronic cigarette smoking. Eur Respir J 2000;15:915-21.
Palmer RM, Wilson RF, Hasan AS, Scott DA. Mechanisms of action of environmental factors – Tobacco smoking. J Clin Periodontol 2005;32 Suppl 6:180-95.
Ah MK, Johnson GK, Kaldahl WB, Patil KD, Kalkwarf KL. The effect of smoking on the response to periodontal therapy. J Clin Periodontol 1994;21:91-7.
Haber J, Wattles J, Crowley M, Mandell R, Joshipura K, Kent RL, et al.
Evidence for cigarette smoking as a major risk factor for periodontitis. J Periodontol 1993;64:16-23.
Ketabi M, Hirsch RS. The effects of local anesthetic containing adrenaline on gingival blood flow in smokers and non-smokers. J Clin Periodontol 1997;24:888-92.
Calsina G, Ramón JM, Echeverría JJ. Effects of smoking on periodontal tissues. J Clin Periodontol 2002;29:771-6.
Lindhe J, Brånemark PI. Changes in microcirculation after local application of sex hormones. J Periodontal Res 1967;2:185-93.
Lindhe J, Brånemark PI. Changes in vascular permeability after local application of sex hormones. J Periodontal Res 1967;2:259-65.
Machtei EE, Mahler D, Sanduri H, Peled M. The effect of menstrual cycle on periodontal health. J Periodontol 2004;75:408-12.
Mogi M, Otogoto J. Expression of cathepsin-K in gingival crevicular fluid of patients with periodontitis. Arch Oral Biol 2007;52:894-8.
Garg G, Thorat MK, Pradeep AR, Daisy H, Hadge P. Correlation of gingival crevicular fluid levels of cathepsin k and oncostatin m in periodontal health and disease. AOSR 2012;2:60-6.
Garg G, Pradeep AR, Thorat MK. Effect of nonsurgical periodontal therapy on crevicular fluid levels of Cathepsin K in periodontitis. Arch Oral Biol 2009;54:1046-51.
Cesar-Neto JB, Duarte PM, de Oliveira MC, Tambeli C H, Sallum EA, Nociti FH Jr. Smoking modulates interleukin-6: interleukin-10 and RANKL: osteoprotegerin ratios in the periodontal tissues. Journal of Periodontal Research 2007:42:184-91.
Boström L, Linder LE, Bergström J. Smoking and crevicular fluid levels of IL-6 and TNF-alpha in periodontal disease. J Clin Periodontol 1999;26:352-7.
Kudo O, Fujikawa Y, Itonaga I, Sabokbar A, Torisu T, Athanasou NA, et al.
Proinflammatory cytokine (TNF alpha/IL-1alpha) induction of human osteoclast formation. J Pathol 2002;198:220-7.
Teng YT. Protective and destructive immunity in the periodontium: Part 2 – T-cell-mediated immunity in the periodontium. J Dent Res 2006;85:209-19.
Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature 2003;423:337-42.
Preber H, Bergström J. Cigarette smoking in patients referred for periodontal treatment. Scand J Dent Res 1986;94:102-8.
Torrungruang K, Nisapakultorn K, Sutdhibhisal S, Tamsailom S, Rojanasomsith K, Vanichjakvong O, et al.
The effect of cigarette smoking on the severity of periodontal disease among older Thai adults. J Periodontol 2005;76:566-72.
Feldman RS, Bravacos JS, Rose CL. Association between smoking different tobacco products and periodontal disease indexes. J Periodontol 1983;54:481-7.
Lie MA, van der Weijden GA, Timmerman MF, Loos BG, van Steenbergen TJ, van der Velden U, et al.
Oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis. J Clin Periodontol 1998;25:677-86.
Danielsen B, Manji F, Nagelkerke N, Fejerskov O, Baelum V. Effect of cigarette smoking on the transition dynamics in experimental gingivitis. J Clin Periodontol 1990;17:159-64.
Preber H, Kant T, Bergström J. Cigarette smoking, oral hygiene and periodontal health in Swedish army conscripts. J Clin Periodontol 1980;7:106-13.
Dr. Priya Lochana Gajendran
Department of Periodontology, Saveetha Dental College, No. 162, Poonamalee High Road, Chennai - 600 077, Tamil Nadu
Source of Support: None, Conflict of Interest: None
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5]