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ORIGINAL RESEARCH Table of Contents   
Year : 2013  |  Volume : 24  |  Issue : 3  |  Page : 302-308
A comparative study of various decalcification techniques


1 Department of Oral Pathology, College of Dentistry, Gulf Medical University, Ajman, United Arab Emirates
2 College of Dental Sciences, Davangere, Karnataka, India

Correspondence Address:
Prathibha Prasad
Department of Oral Pathology, College of Dentistry, Gulf Medical University, Ajman
United Arab Emirates
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0970-9290.117991

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Background : Study of fibrilar, cellular and sub cellular structures of mineralized tissues is only possible after the removal of the calcium apatite of these tissues by the process of demineralization. Aims: The present study aims to evaluate six commonly used demineralizing agents to identify the best decalcifying agent. Materials and Methods: The present study included six different decalcifying solutions: 10% formal nitric acid, 8% formal nitric acid, 10% formic acid, 8% formic acid, Perenyi's fluid and Ethylene Di-Amine Tetra Acetic Acid. eight samples of posterior mandible of rat were decalcified in each of the decalcifying solutions and subjected to chemical end-point test. Ehrlich's Hematoxylin stain was used. Statistical Analysis Used: One way ANOVA was used for multiple group comparisons and Chi-square test was used for analyzing categorical data. P value of 0.05/less was set for statistical significance. Results: Samples treated with EDTA showed the best overall histological impression and the tissue integrity were well preserved. Formal nitric of both the percentages 10 and 8% gave fairly good cellular detail and were rapid in their action. Conclusion: The final impression led to the proposition that EDTA was indeed the best decalcifying agent available. However, with time constraint, the use of formal nitric acid is advocated.


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